8FA6
Crystal structure of Ky15.10 Fab in complex with circumsporozoite protein DND peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.549, 61.905, 82.540 |
| Unit cell angles | 90.00, 104.49, 90.00 |
Refinement procedure
| Resolution | 29.380 - 2.600 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.24060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.554 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 29115 | 1273 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.989 | 0.784 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5, 25% PEG3350 |
