8FA5
Crystal structure of Xanthomonas campestris GH35 beta-galactosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.865, 84.768, 99.811 |
Unit cell angles | 90.00, 96.61, 90.00 |
Refinement procedure
Resolution | 42.793 - 1.700 |
R-factor | 0.1712 |
Rwork | 0.170 |
R-free | 0.20260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.227 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (v1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 283540 | 27563 |
<I/σ(I)> | 13.7 | |
Completeness [%] | 97.6 | |
Redundancy | 4.3 | |
CC(1/2) | 0.990 | 0.610 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.2M lithium chloride, 20% PEG 3350 |