8F9S
Crystal structure of Ky15.2 Fab in complex with circumsporozoite protein NDN peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033192 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.479, 77.853, 123.649 |
| Unit cell angles | 90.00, 91.68, 90.00 |
Refinement procedure
| Resolution | 39.260 - 2.100 |
| R-factor | 0.2063 |
| Rwork | 0.205 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.614 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.130 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 50174 | 2056 |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.998 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M ammonium fluoride, 20% (w/v) PEG3350 |
