8F9R

Rabbit sialic acid esterase (SIAE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	114.070, 152.690, 81.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.970
R-factor	0.2104
Rwork	0.207
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.674
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.060
High resolution limit [Å]	2.950	2.950
Rmeas	0.184
Number of reflections	59316	5810
<I/σ(I)>	14.3
Completeness [%]	100.0
Redundancy	11.6
CC(1/2)		0.314

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M ammonium phosphate, 0.1 M Tris, pH 8.5, 50% v/v MPD