8F9Q

Guinea pig sialic acid esterase (SIAE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 64
Unit cell lengths	206.554, 206.554, 155.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.610 - 2.760
R-factor	0.188
Rwork	0.188
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.797
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.760	2.760
Rmeas	0.582
Number of reflections	192756	19184
<I/σ(I)>	9
Completeness [%]	100.0
Redundancy	20.5
CC(1/2)		0.259

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M MES, pH 6.5, 10% w/v PEG5000 MME, 12% v/v 1-propanol