8F9F

Crystal structure of Ky15.2 Fab in complex with circumsporozoite protein NANP3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033192
Spacegroup name	P 21 21 21
Unit cell lengths	77.372, 86.574, 134.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.730 - 2.200
R-factor	0.2142
Rwork	0.213
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.690
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.230
High resolution limit [Å]	2.200	2.200
Number of reflections	46439	1823
<I/σ(I)>	11.2
Completeness [%]	99.7
Redundancy	13.4
CC(1/2)	0.998	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M magnesium acetate, 20% (w/v) PEG3350