8F9E
Crystal structure of Ky15.2 Fab in complex with circumsporozoite protein KQPA peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033192 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.167, 85.209, 133.475 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.440 - 2.950 |
R-factor | 0.2177 |
Rwork | 0.216 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.571 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 19407 | 934 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.8 | |
Redundancy | 13.3 | |
CC(1/2) | 0.992 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES pH 7.5, 25% (w/v) PEG3350, 0.2 M sodium chloride |