8F8Z

PHF2 (PHD+JMJ) in Complex with H3 Histone N-Terminal Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	73.854, 83.756, 104.128
Unit cell angles	90.00, 103.67, 90.00

Refinement procedure

Resolution	54.500 - 3.300
R-factor	0.2154
Rwork	0.213
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.421
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.500	3.400
High resolution limit [Å]	3.300	3.300
Number of reflections	36233	3642
<I/σ(I)>	3.4	0.9
Completeness [%]	98.6	91.5
Redundancy	7.8
CC(1/2)	0.929	0.434

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	292	2.5M Ammonium Sulfate 90mM Bis-Tris Propane, pH 6.3 4% Pentaerythritol ethoxylate (3/4 EO/OH)