8F8E

Crystal structure of the WDR domain of human DCAF1 in complex with OICR-8268 compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	48.865, 88.084, 73.562
Unit cell angles	90.00, 97.56, 90.00

Refinement procedure

Resolution	44.080 - 1.550
R-factor	0.1818
Rwork	0.181
R-free	0.20898
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pxw
RMSD bond length	0.007
RMSD bond angle	1.421
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.010	1.580
High resolution limit [Å]	1.550	4.210	1.550
Rmerge	0.065	0.044	0.748
Rmeas	0.072
Rpim	0.031
Number of reflections	88118	3396	4407
<I/σ(I)>	27.6		1.88
Completeness [%]	98.4	98	98.7
Redundancy	5.3	5.5	5.1
CC(1/2)	0.999		0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG3350, 0.2M di-Ammonium Citrate