8F73
Crystal structure of Pseudomonas aeruginosa UDP-glucose phosphorylase in complex with UDP-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.953722 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.715, 114.847, 155.761 |
Unit cell angles | 90.00, 97.39, 90.00 |
Refinement procedure
Resolution | 46.980 - 2.900 |
R-factor | 0.1968 |
Rwork | 0.195 |
R-free | 0.23570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3juk |
RMSD bond length | 0.007 |
RMSD bond angle | 0.997 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.520 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.109 | 0.860 |
Rpim | 0.580 | |
Number of reflections | 65768 | 4608 |
<I/σ(I)> | 9.3 | 1.8 |
Completeness [%] | 99.7 | |
Redundancy | 3.9 | |
CC(1/2) | 0.960 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate trihydrate pH 4.6 2.0 M sodium formate |