8F6V
Crystal Structure of Nanobody VHH108 Bound to Its Antigen PA14 Cif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979312 |
Spacegroup name | P 61 |
Unit cell lengths | 98.995, 98.995, 196.987 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.230 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.086 | 0.783 |
Rmeas | 0.103 | 1.004 |
Rpim | 0.056 | 0.621 |
Number of reflections | 48028 | 4792 |
<I/σ(I)> | 7.36 | 1.28 |
Completeness [%] | 99.3 | 99.79 |
Redundancy | 2.9 | 2.4 |
CC(1/2) | 0.991 | 0.425 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 292.8 | Morpheus I Condition G2 (composition by Molecular Dimensions stock solutions with catalogue numbers listed at end) 20% (v/v) ethylene glycol, 10% (w/v) PEG8000, 20 mM sodium formate, 20 mM ammonium acetate, 20 mM sodium citrate tribasic dihydrate, 20 mM potassium sodium tartrate tetrahydrate, 20 mM sodium oxamate, 100 mM imidazole/MES monohydrate (acid) pH 6.5 Stock mixtures listed below with catalogue number in brackets. 30% (v/v) Precipitant Mix 2 [MD2-100-82], 100 mM carboxylic acids mix [MD2-100-76], 100 mM Buffer System 1 pH 6.5 [MD2-100-100] |