8F6D

Crystal structure of the CNNM2 CBS-pair domain in complex with ARL15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	66.018, 73.323, 79.327
Unit cell angles	94.35, 95.50, 115.33

Refinement procedure

Resolution	29.530 - 3.200
R-factor	0.2655
Rwork	0.264
R-free	0.29270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iy0
RMSD bond length	0.004
RMSD bond angle	0.791
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	20526	1986
<I/σ(I)>	10.4	0.6
Completeness [%]	94.2	93.1
Redundancy	3.7	3.8
CC(1/2)	0.998	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2 M sodium chloride, 0.1 M Tris pH 8.5, 25% (w/v) PEG3350, 0.5 mM GppNp