8F69
Crystal structure of murine PolG2 dimer bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2017-12-02 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 88.027, 88.027, 343.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.630 - 2.200 |
R-factor | 0.1949 |
Rwork | 0.194 |
R-free | 0.22830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g5i |
RMSD bond length | 0.004 |
RMSD bond angle | 0.633 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.630 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.127 | 1.490 |
Number of reflections | 69448 | 6754 |
<I/σ(I)> | 20.59 | 1.1 |
Completeness [%] | 99.7 | 99.16 |
Redundancy | 8.9 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% MPD 0.1 M MES pH 6.5 |