8F5N
Identification of an Immunodominant region on a Group A Streptococcus T-antigen Reveals Temperature-Dependent Motion in Pili
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2017-08-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95372 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 126.362, 41.112, 171.600 |
Unit cell angles | 90.00, 110.42, 90.00 |
Refinement procedure
Resolution | 41.900 - 1.900 |
R-factor | 0.20377 |
Rwork | 0.202 |
R-free | 0.24358 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6n0a |
RMSD bond length | 0.009 |
RMSD bond angle | 1.621 |
Data reduction software | XDS (VERSION Jun 1, 2017) |
Data scaling software | Aimless (1.17.29) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.870 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.077 | 1.425 |
Rmeas | 0.084 | |
Rpim | 0.032 | 0.583 |
Number of reflections | 65913 | 4228 |
<I/σ(I)> | 11.8 | 1.2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 6.8 | 6.9 |
CC(1/2) | 0.999 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 0.3 Mdiethyleneglycol, 0.3 Mtriethyleneglycol, 0.3 M tetraethyleneglycol, 0.3 Mpentaethyleneglycol and 0.1 M MOPS/HEPES-Na pH 7.5 |