8F5M

Crystal structure of P74 gp62

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98756
Spacegroup name	C 2 2 21
Unit cell lengths	110.937, 210.102, 181.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.400
R-factor	0.18836
Rwork	0.186
R-free	0.23146
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.474
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	REFMAC (refmac5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.380	2.380
Rmerge	0.107	1.295
Number of reflections	84905	4103
<I/σ(I)>	13.7
Completeness [%]	100.0
Redundancy	8
CC(1/2)	0.998	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	20-30 % PEG 3350, 100 mM KCl