8F5M
Crystal structure of P74 gp62
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98756 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.937, 210.102, 181.089 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.400 |
R-factor | 0.18836 |
Rwork | 0.186 |
R-free | 0.23146 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.474 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | REFMAC (refmac5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.420 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.107 | 1.295 |
Number of reflections | 84905 | 4103 |
<I/σ(I)> | 13.7 | |
Completeness [%] | 100.0 | |
Redundancy | 8 | |
CC(1/2) | 0.998 | 0.738 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 273 | 20-30 % PEG 3350, 100 mM KCl |