8F5G
NusG-RNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.977 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 68.951, 87.451, 148.793 |
Unit cell angles | 90.00, 103.24, 90.00 |
Refinement procedure
Resolution | 48.280 - 2.700 |
R-factor | 0.2122 |
Rwork | 0.209 |
R-free | 0.25580 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.476 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.280 | 2.790 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.048 | 0.273 |
Number of reflections | 23756 | 2379 |
<I/σ(I)> | 18.7 | |
Completeness [%] | 99.8 | |
Redundancy | 12.5 | |
CC(1/2) | 0.997 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 294.15 | 0.25 M sodium acetate, pH 5.2, 12% w/v PEG3350 |