8F5G

NusG-RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	C 1 2 1
Unit cell lengths	68.951, 87.451, 148.793
Unit cell angles	90.00, 103.24, 90.00

Refinement procedure

Resolution	48.280 - 2.700
R-factor	0.2122
Rwork	0.209
R-free	0.25580
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.476
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.280	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.048	0.273
Number of reflections	23756	2379
<I/σ(I)>	18.7
Completeness [%]	99.8
Redundancy	12.5
CC(1/2)	0.997	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	294.15	0.25 M sodium acetate, pH 5.2, 12% w/v PEG3350