8F2W

Structure of a B-Form Dodecamer: 5'-CGCGAATTCGCG-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	25.580, 40.104, 66.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.290 - 1.300
R-factor	0.1894
Rwork	0.186
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bna
RMSD bond length	0.011
RMSD bond angle	1.444
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.300	1.350
High resolution limit [Å]	1.300	1.300
Number of reflections	16736	1649
<I/σ(I)>	16.74
Completeness [%]	95.2
Redundancy	1.9
CC(1/2)	0.981	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	MPD, SODIUM CACODYLATE TRIHYDRATE, SPERMINE TETRACHLORIDE, SODIUM CHLORIDE, MAGNESIUM HEXAHYDRATE