8F2Q
Human Parvovirus B19 Nonstructural NS1 Protein NLS bound to Importin Alpha 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95373 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.197, 90.188, 100.512 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.540 - 2.700 |
R-factor | 0.2154 |
Rwork | 0.215 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uaf |
RMSD bond length | 0.003 |
RMSD bond angle | 0.576 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.540 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.160 | 1.045 |
Number of reflections | 20091 | 2620 |
<I/σ(I)> | 6.3 | 1.6 |
Completeness [%] | 99.8 | |
Redundancy | 3.8 | |
CC(1/2) | 0.947 | 0.563 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 0.7M sodium citrate, 0.1M HEPES pH6.5, 0.01M DTT |