8F2Q

Human Parvovirus B19 Nonstructural NS1 Protein NLS bound to Importin Alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	78.197, 90.188, 100.512
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.540 - 2.700
R-factor	0.2154
Rwork	0.215
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uaf
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.540	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.160	1.045
Number of reflections	20091	2620
<I/σ(I)>	6.3	1.6
Completeness [%]	99.8
Redundancy	3.8
CC(1/2)	0.947	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.7M sodium citrate, 0.1M HEPES pH6.5, 0.01M DTT