8F2H
Wild type P53 dimer structure from human cancer cells
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | TFS TALOS F200C |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI CETA (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.2 |
| Number of Particle | 8000 |
| Software | RELION,PHENIX,ISOLDE |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING ONLY |
