8F1B
Structure of zinc-bound ZrgA deletion 124-184 from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.991, 66.914, 88.242 |
| Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
| Resolution | 46.670 - 2.410 |
| R-factor | 0.22012 |
| Rwork | 0.218 |
| R-free | 0.26196 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ezw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.323 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.1.018) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 2.500 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Number of reflections | 36368 | 3798 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.996 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.6 | 296 | Protein at 10 mg/mL was combined in a 1:1 ratio with precipitant solution containing 0.1 M sodium acetate pH 4.6, 0.2 M ammonium acetate, 27% PEG 4000 |
