8F16
Structure of the STUB1 TPR domain in complex with H203, an all-D Helicon Polypeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.18057 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.560, 66.880, 103.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.410 - 1.560 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q49 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.410 | 1.590 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.030 | 0.676 |
Number of reflections | 50341 | |
<I/σ(I)> | 22.4 | 2.2 |
Completeness [%] | 99.5 | 100 |
Redundancy | 6.3 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M BICINE pH 8.5, 15% w/v PEG 1,500 |