8F12

Structure of the MDM2 P53 binding domain in complex with H103, an all-D Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03317
Spacegroup name	P 43 21 2
Unit cell lengths	60.310, 60.310, 84.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.110 - 1.860
R-factor	0.2162
Rwork	0.215
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g03
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.110	1.900
High resolution limit [Å]	1.860	1.860
Rmerge		1.577
Rmeas	0.113
Number of reflections	13715	817
<I/σ(I)>	18
Completeness [%]	99.8	98.2
Redundancy	25.8	23.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	3.2 M Ammonium sulfate, 0.1 M Citrate pH 5.0