8F10
Structure of the MDM2 P53 binding domain in complex with H102, an all-D Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.150, 39.920, 42.360 |
| Unit cell angles | 90.00, 95.44, 90.00 |
Refinement procedure
| Resolution | 28.020 - 1.280 |
| R-factor | 0.1546 |
| Rwork | 0.154 |
| R-free | 0.16690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g03 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.170 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.049 | 0.420 |
| Number of reflections | 20614 | 233 |
| <I/σ(I)> | 18.2 | 2.1 |
| Completeness [%] | 85.3 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.01 M tri-Sodium citrate, 33 % w/v PEG 6000 |
