8F0Z
Structure of the MDM2 P53 binding domain in complex with H101, an all-D Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 40.871, 81.357, 34.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.870 - 1.610 |
| Rwork | 0.221 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g03 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.870 | 1.640 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.067 | |
| Number of reflections | 15696 | 774 |
| <I/σ(I)> | 19.3 | 2.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 12.8 | 12.9 |
| CC(1/2) | 0.998 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 3.5 M Sodium Formate pH 7.0 |
