8F0V

Lipocalin-like Milk protein-2 - E38A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2022-05-07
Detector	RDI CMOS_8M
Wavelength(s)	1.07
Spacegroup name	P 2 21 21
Unit cell lengths	39.771, 52.337, 136.348
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.180 - 2.951
Rwork	0.234
R-free	0.29360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bkx
RMSD bond length	0.010
RMSD bond angle	1.361
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.180	3.130
High resolution limit [Å]	2.950	2.950
Rmerge	0.129	2.000
Rpim	0.054	0.830
Number of reflections	6461	1021
<I/σ(I)>	11.5	1
Completeness [%]	99.9	100
Redundancy	6.6	6.9
CC(1/2)	0.998	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0 (0.002 M Zinc sulfate heptahydrate; 0.08 M HEPES pH 7.0; 25 % v/v Jeffamine