8F0V
Lipocalin-like Milk protein-2 - E38A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2022-05-07 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.07 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 39.771, 52.337, 136.348 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.180 - 2.951 |
Rwork | 0.234 |
R-free | 0.29360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bkx |
RMSD bond length | 0.010 |
RMSD bond angle | 1.361 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.180 | 3.130 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.129 | 2.000 |
Rpim | 0.054 | 0.830 |
Number of reflections | 6461 | 1021 |
<I/σ(I)> | 11.5 | 1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.6 | 6.9 |
CC(1/2) | 0.998 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0 (0.002 M Zinc sulfate heptahydrate; 0.08 M HEPES pH 7.0; 25 % v/v Jeffamine |