8EZX

Lysozyme Anomalous Dataset at 293 K and 7.1 keV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-01-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.7462
Spacegroup name	P 43 21 2
Unit cell lengths	77.706, 77.706, 37.121
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.883 - 1.900
Rwork	0.130
R-free	0.16640
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.565
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.883	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.033	0.119
Rpim	0.022	0.084
Number of reflections	8682	2347
<I/σ(I)>	30.7	9.9
Completeness [%]	92.9	78.8
Redundancy	5.6	5
CC(1/2)	0.997	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	5 uL of 0.6 M Sodium Chloride in 100 mM Sodium Acetate buffer pH 4.5 and 25% ethylene glycol were mixed with 5 uL 100 mg/mL lysozyme in 100 mM Sodium Acetate.