8EZW

Structure of Apo ZrgA deletion 124-184 from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	81.055, 65.444, 88.735
Unit cell angles	90.00, 98.77, 90.00

Refinement procedure

Resolution	45.880 - 2.000
R-factor	0.19425
Rwork	0.192
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ahw
RMSD bond length	0.016
RMSD bond angle	1.847
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (7.1.018)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.180	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.056	0.706
Rmeas	0.065	0.820
Rpim	0.033	0.410
Total number of observations	236374	16684
Number of reflections	62935	4390
<I/σ(I)>	14.1	2.1
Completeness [%]	99.0
Redundancy	3.8	3.8
CC(1/2)	0.999	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	5.5	296	Protein at 10 mg/mL was combined at a 1:1 ratio with precipitant solution containing 0.2 M NaCl, 0.1 M bis-tris propane pH 5.5, 28% PEG 3350