8EZS

Crystal structure of the HipS(Lp)-HipT(Lp) complex from Legionella pneumophila, Sel-met protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 41 21 2
Unit cell lengths	44.854, 44.854, 392.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.470
R-factor	0.2054
Rwork	0.203
R-free	0.26010
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.533
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.084	0.508
Rpim	0.029	0.408
Number of reflections	14111	211
<I/σ(I)>	19.5	1.21
Completeness [%]	89.1	37.4
Redundancy	10.9	1.6
CC(1/2)	0.994	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M KCl, 10 mM Tris pH 7, 20% PEG 4K, SeU soak, cryo paratone