8EZR

Crystal structure of the HipS(Lp)-HipT(Lp) complex from Legionella pneumophila, native protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 41 21 2
Unit cell lengths	45.038, 45.038, 393.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.830 - 1.950
R-factor	0.2155
Rwork	0.213
R-free	0.26140
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.711
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.095	1.035
Rpim	0.024	0.326
Number of reflections	29985	1222
<I/σ(I)>	53.2	1.53
Completeness [%]	95.5	81.2
Redundancy	14.4
CC(1/2)	1.000	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	30% PEG 1K, 0.1 M Hepes pH 7.5, cryo paratone