8EYU
Structure of Beetroot dimer bound to DFAME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.937, 50.742, 95.555 |
Unit cell angles | 90.00, 94.08, 90.00 |
Refinement procedure
Resolution | 47.660 - 1.950 |
R-factor | 0.1809 |
Rwork | 0.178 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5bjo |
RMSD bond length | 0.004 |
RMSD bond angle | 0.818 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.660 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.056 | |
Rmeas | 0.061 | |
Number of reflections | 21035 | 2095 |
<I/σ(I)> | 16.32 | |
Completeness [%] | 99.8 | |
Redundancy | 6.5 | |
CC(1/2) | 0.985 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294.15 | 2 M Ammonium sulfate, 5% 2-propanol |