8EYS
X-ray crystal structure of salmonella typhimurium Tryptophan synthase internal aldimine at pH 5.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2022-09-06 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 184.659, 61.810, 67.733 |
Unit cell angles | 90.00, 94.74, 90.00 |
Refinement procedure
Resolution | 16.540 - 2.200 |
Rwork | 0.163 |
R-free | 0.19730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bks |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.580 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.110 | |
Number of reflections | 37370 | 5337 |
<I/σ(I)> | 11.6 | |
Completeness [%] | 96.5 | |
Redundancy | 6.9 | |
CC(1/2) | 0.997 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 50 mM Bicine pH 7.8, 1 mM EDTA, 0.2 mM PLP, 2 mM spermine, and 6% PEG 8000; acidified with sodium acetate pH 5.0 |