8EY6
LaM domain of human LARP1 in complex with AAAAAG RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-15 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.03321 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.523, 46.187, 58.895 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.340 - 1.630 |
R-factor | 0.2105 |
Rwork | 0.209 |
R-free | 0.24220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7soo |
RMSD bond length | 0.007 |
RMSD bond angle | 0.982 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.660 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 12601 | 629 |
<I/σ(I)> | 16.6 | 1.5 |
Completeness [%] | 97.2 | 96.3 |
Redundancy | 6 | 4.8 |
CC(1/2) | 1.000 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M Bis-Tris pH 6.5, 2 M ammonium sulfate |