8EXZ

Structure of GDAP1 containing CMT mutant T157P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	73.586, 80.064, 85.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.860 - 2.820
R-factor	0.2572
Rwork	0.255
R-free	0.27920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iuh
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.540	2.869
High resolution limit [Å]	2.820	2.820
Rmerge	0.126	0.708
Rpim		0.352
Number of reflections	12544	618
<I/σ(I)>	9	2.2
Completeness [%]	98.1	98.6
Redundancy	4.9	4.6
CC(1/2)	0.998	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.2M Ammonium Sulfate, 0.1M Bis-Tris, 25% w/v PEG 3350