8EXW

Drosophila melanogaster indirect flight muscle myosin II (subfragment-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9999
Spacegroup name	P 1 21 1
Unit cell lengths	42.394, 126.806, 92.581
Unit cell angles	90.00, 90.13, 90.00

Refinement procedure

Resolution	43.480 - 2.500
R-factor	0.1908
Rwork	0.188
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	various
RMSD bond length	0.003
RMSD bond angle	0.524
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BALBES
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.540
High resolution limit [Å]	2.500	2.500
Rmeas	0.064
Rpim	0.033
Number of reflections	31441	1630
<I/σ(I)>	11.1
Completeness [%]	99.1
Redundancy	3.7
CC(1/2)		0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	80% Hampton Research Index screen H11: 0.1 M Potassium thiocyanate, 30 %w/v Polyethylene glycol monomethyl ether 2,000 20% Hampton Research additive screen C3: 0.1 M Phenol