8EXF

Crystal structure of human FAM46A-BCCIPa complex at 3.2 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	91.621, 188.145, 93.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.040 - 3.220
R-factor	0.2092
Rwork	0.207
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w36
RMSD bond length	0.004
RMSD bond angle	0.629
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.070	0.954
Rpim	0.040	0.579
Number of reflections	13367	671
<I/σ(I)>	17.8	1.1
Completeness [%]	98.9	98.2
Redundancy	3.9	3.7
CC(1/2)	0.989	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Tris pH8.0, 0.2 M Sodium thiocyanate, 15-18% PEG 3350.