8EWX
N-terminal WD40 domain of beta'-COPI subunit with two chains in the asymmetric unit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.997, 89.821, 69.070 |
| Unit cell angles | 90.00, 93.69, 90.00 |
Refinement procedure
| Resolution | 27.460 - 1.240 |
| R-factor | 0.1345 |
| Rwork | 0.133 |
| R-free | 0.16100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j73 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.097 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.330 | 1.260 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Number of reflections | 160014 | 5385 |
| <I/σ(I)> | 10.1 | 1.3 |
| Completeness [%] | 95.1 | 64.9 |
| Redundancy | 4.3 | 2.5 |
| CC(1/2) | 0.998 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | PEG4000 |
