8EW9
Crystal structure of Saccharomyces cerevisiae Altered Inheritance rate of Mitochondria protein 46 (AIM46p)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03321 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.870, 71.350, 40.220 |
Unit cell angles | 90.00, 95.17, 90.00 |
Refinement procedure
Resolution | 40.060 - 2.000 |
R-factor | 0.1736 |
Rwork | 0.167 |
R-free | 0.23350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7RSG |
RMSD bond length | 0.007 |
RMSD bond angle | 0.773 |
Data reduction software | XDS (VERSION Jan 26, 2018 BUILT=20180409) |
Data scaling software | XSCALE (VERSION Jan 26, 2018 BUILT=20180409) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.060 | 2.072 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.123 | 1.159 |
Rmeas | 0.134 | 1.253 |
Rpim | 0.051 | 0.473 |
Number of reflections | 15174 | 1471 |
<I/σ(I)> | 11.51 | 1.36 |
Completeness [%] | 99.6 | 98.92 |
Redundancy | 6.8 | 6.9 |
CC(1/2) | 0.998 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Crystals grew from 180 nL 31.4 mg/mL Aim46p Nd62, 20 nL microseeds in 30% PEG 2000, 0.1 M MES buffer, pH 6.0, mixed with 300 nL reservoir solution composed of 18% PEG 2000, 100 mM MES buffer, pH 6.5. Microseeded crystallization plates were set with a Mosquito crystallization robot in a MRC SD2 crystallization plate. Crystals were cryopreserved with reservoir solution supplemented with 30% PEG 2000 |