8EW8
Crystal structure of Saccharomyces cerevisiae Altered Inheritance rate of Mitochondria protein 18 (AIM18p) R123A mutant
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.964370, 0.979370, 0.979610 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 127.390, 127.390, 54.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.700 - 2.150 |
R-factor | 0.1808 |
Rwork | 0.178 |
R-free | 0.21160 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.798 |
Data reduction software | XDS (VERSION Nov 11, 2017 BUILT=20171111) |
Data scaling software | XSCALE (VERSION Nov 11, 2017 BUILT=20171111) |
Phasing software | PHASER (2.8.0) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.700 | 2.227 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.098 | 0.996 |
Rmeas | 0.101 | 1.039 |
Rpim | 0.023 | 0.284 |
Number of reflections | 27153 | 2241 |
<I/σ(I)> | 21.8 | 2.38 |
Completeness [%] | 97.6 | 81.11 |
Redundancy | 18.8 | 12.3 |
CC(1/2) | 0.999 | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | Crystals for data collection were obtained by mixing 200 nL of protein soiution (15.1 mg/mL Nd70 R123A Aim18p, 20 mM HEPES buffer, pH 7.5, 200 mM NaCl, 2 mM TCEP) with 150 nL of reservoir solution (26% PEG 3350, 200 mM Li2SO4, 100 mM HEPES pH 7.5) in a SD2 crystallization tray with a TTP Labtech Mosquito crystallization robot. Crystals were cryoprotected with reservoir solution supplemented to 30% PEG 3350 |