8EVF
HUMAN DNA POLYMERASE ETA EXTENSION COMPLEX WITH AN INCOMING DCTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-17 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.12723 |
Spacegroup name | P 61 |
Unit cell lengths | 98.671, 98.671, 81.736 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.530 - 2.870 |
R-factor | 0.2063 |
Rwork | 0.204 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l1i |
RMSD bond length | 0.004 |
RMSD bond angle | 0.745 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.920 |
High resolution limit [Å] | 2.870 | 2.870 |
Rmerge | 0.106 | 0.690 |
Rpim | 0.049 | |
Number of reflections | 10308 | 536 |
<I/σ(I)> | 15.68 | 1.98 |
Completeness [%] | 98.5 | 99.3 |
Redundancy | 5.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M MES PH 5.5, 5mM calcium chloride, 15-22% PEG 2000 MME |