8EVE
HUMAN DNA POLYMERASE ETA INSERTION COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-17 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.12723 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.990, 98.990, 81.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.400 - 2.350 |
| R-factor | 0.1917 |
| Rwork | 0.189 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l1i |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 2.440 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.087 | |
| Rmeas | 0.227 | 0.674 |
| Rpim | 0.064 | 0.499 |
| Number of reflections | 18885 | 1397 |
| <I/σ(I)> | 5.3 | 2.1 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 10.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1 M MES PH 5.5, 5mM Calcium Chloride, 15-22% PEG 2000 MME |
