8EVD
Crystal Structure of Nanobody VHH101 Bound to Its Antigen PA14 Cif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979339 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 188.960, 91.990, 151.215 |
Unit cell angles | 90.00, 92.54, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.190 | 2.071 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.082 | 0.902 |
Rmeas | 0.096 | 1.054 |
Rpim | 0.049 | 0.530 |
Number of reflections | 165194 | 16964 |
<I/σ(I)> | 10.57 | 2.02 |
Completeness [%] | 94.5 | 97.76 |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 0.997 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292.8 | 15% (v/v) isopropanol, 1 M ammonium citrate/ammonium hydroxide pH 8.5 |