8EV4
NlpC B3 - Trichomonas Vaginalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 34.050, 34.050, 181.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.470 - 1.300 |
| R-factor | 0.14823 |
| Rwork | 0.147 |
| R-free | 0.16827 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bim |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.470 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.019 | 0.394 |
| Rmeas | 0.027 | 0.557 |
| Rpim | 0.019 | 0.394 |
| Number of reflections | 27581 | 2662 |
| <I/σ(I)> | 13.7 | 1.5 |
| Completeness [%] | 99.9 | 99.51 |
| Redundancy | 2 | 2 |
| CC(1/2) | 1.000 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 1.26 M Ammonium Sulfate, 0.1 M Tris pH 8.5. |
