8ETD
Crystal Structure of Schizosaccharomyces pombe Rho1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-02-12 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.691, 66.349, 75.551 |
| Unit cell angles | 90.00, 112.78, 90.00 |
Refinement procedure
| Resolution | 39.140 - 2.780 |
| R-factor | 0.1932 |
| Rwork | 0.191 |
| R-free | 0.23230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zvp |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.830 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.200 | 0.820 |
| Number of reflections | 12478 | 617 |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.950 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Sodium citrate tribasic tetrahydrate (pH 5.6), 1.9 M Ammonium sulfate |
