8ESD
Crystal structure of COMMD7-COMMD9-COMMD5-COMMD10 tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 215.191, 74.895, 63.632 |
| Unit cell angles | 90.00, 96.40, 90.00 |
Refinement procedure
| Resolution | 38.500 - 3.330 |
| R-factor | 0.242 |
| Rwork | 0.238 |
| R-free | 0.27830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bp6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.520 | 3.350 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rpim | 0.080 | 0.908 |
| Number of reflections | 16930 | 2673 |
| <I/σ(I)> | 6.2 | 0.9 |
| Completeness [%] | 96.5 | |
| Redundancy | 5.1 | |
| CC(1/2) | 0.997 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 2 uM crown ether and 10% glycerol and grown in 22% ethanol and 5 mM EDTA |
