8ESD

Crystal structure of COMMD7-COMMD9-COMMD5-COMMD10 tetramer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	C 1 2 1
Unit cell lengths	215.191, 74.895, 63.632
Unit cell angles	90.00, 96.40, 90.00

Refinement procedure

Resolution	38.500 - 3.330
R-factor	0.242
Rwork	0.238
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bp6
RMSD bond length	0.003
RMSD bond angle	0.922
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.520	3.350
High resolution limit [Å]	3.130	3.130
Rpim	0.080	0.908
Number of reflections	16930	2673
<I/σ(I)>	6.2	0.9
Completeness [%]	96.5
Redundancy	5.1
CC(1/2)	0.997	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2 uM crown ether and 10% glycerol and grown in 22% ethanol and 5 mM EDTA