8ERW

Precisely patterned nanofibers made from extendable protein multiplexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	55.318, 55.380, 145.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.990 - 2.880
R-factor	0.2641
Rwork	0.261
R-free	0.28640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Computational design model
Data reduction software	HKL-2000 (2.3.10)
Data scaling software	HKL-2000 (2.3.10)
Phasing software	PHASER (2.7.18)
Refinement software	PHENIX (1.19.1-4122-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.940
High resolution limit [Å]	2.880	2.880
Rmeas	0.125	0.776
Rpim	0.055	0.386
Number of reflections	10228	134
<I/σ(I)>	12.6	1.18
Completeness [%]	96.9	89.2
Redundancy	4.4	3.4
CC(1/2)		0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	1:1 mixture of protein solution and crystallization solution consisting of 0.1M Tris-HCl pH 8.5, 25%(w/v) PEG 3000