8ERH

HTLV-1 capsid protein C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-08
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	56.982, 94.121, 96.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.720 - 1.470
R-factor	0.1691
Rwork	0.168
R-free	0.19250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2 prediction
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.000	49.000	1.490
High resolution limit [Å]	1.470	8.040	1.470
Rmerge	0.072	0.037	1.332
Rmeas	0.075	0.038	1.390
Rpim	0.021	0.011	0.386
Total number of observations	1179696	7052	48334
Number of reflections	88526	631	3990
<I/σ(I)>	15.4	45	1.4
Completeness [%]	99.6	98.9	92.1
Redundancy	13.3	11.2	12.1
CC(1/2)	0.999	0.999	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	1.5 M (NH4)2SO4, 0.1 M Na2HPO4, pH 4.2