8EQW
Crystal structure of Fub7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-01-28 |
| Detector | RAYONIX MX-325 |
| Wavelength(s) | 1.1950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.928, 195.295, 112.424 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 47.970 - 1.760 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Alphafold model |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.140 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 396744 | 19735 |
| <I/σ(I)> | 43.36 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 18.1 | 14.7 |
| CC(1/2) | 0.992 | 0.388 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium malonate pH 5.6, 22% PEG 3350 |
