8EQK
Human PU.1 ETS-Domain (165-270) Bound to d(AATAACCGGAAGTGGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-28 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.920105 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.115, 60.640, 44.456 |
Unit cell angles | 90.00, 116.17, 90.00 |
Refinement procedure
Resolution | 23.870 - 1.450 |
R-factor | 0.1518 |
Rwork | 0.150 |
R-free | 0.17940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8e3k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.115 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.870 | 1.502 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.044 | 0.452 |
Rmeas | 0.049 | 0.499 |
Rpim | 0.021 | 0.210 |
Number of reflections | 36361 | 3588 |
<I/σ(I)> | 16.87 | 2.64 |
Completeness [%] | 99.5 | 99.2 |
Redundancy | 5.2 | 5.4 |
CC(1/2) | 0.999 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100 mM Sodium Acetate, pH=4.6, 2% PEG 3350 |