8EQC

Crystal structure of human anti-N1 neuraminidase 3H03 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03321
Spacegroup name	P 21 21 21
Unit cell lengths	75.299, 117.721, 136.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.410 - 2.200
R-factor	0.181
Rwork	0.179
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kmt 6obz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.410	2.240
High resolution limit [Å]	2.200	2.200
Rpim	0.070	0.500
Number of reflections	61480	2759
<I/σ(I)>	11.1	1
Completeness [%]	98.9
Redundancy	5.1
CC(1/2)	0.979	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M (NH4)2PO4, 20% PEG3350