8EQ6
PD1 signaling receptor bound to FAB Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-17 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.744, 78.510, 102.021 |
| Unit cell angles | 90.00, 106.42, 90.00 |
Refinement procedure
| Resolution | 49.616 - 1.650 |
| Rwork | 0.226 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k0y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.922 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.620 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.650 |
| Rmerge | 0.156 | 2.329 |
| Rmeas | 0.169 | 2.657 |
| Rpim | 0.066 | 1.246 |
| Number of reflections | 62817 | 10635 |
| <I/σ(I)> | 4.86 | 0.2 |
| Completeness [%] | 98.9 | 86.2 |
| Redundancy | 6.3 | 4.1 |
| CC(1/2) | 0.994 | 0.338 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 20.5% PEG 3350, 0.4M MgCl2, 0.1M Bis-Tris pH 5.5 |
