8EQ6
PD1 signaling receptor bound to FAB Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-17 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 66.744, 78.510, 102.021 |
Unit cell angles | 90.00, 106.42, 90.00 |
Refinement procedure
Resolution | 49.616 - 1.650 |
Rwork | 0.228 |
R-free | 0.26420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6k0y |
RMSD bond length | 0.009 |
RMSD bond angle | 1.599 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.620 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.156 | 2.329 |
Rmeas | 0.169 | 2.657 |
Rpim | 0.066 | 1.246 |
Number of reflections | 60066 | 10635 |
<I/σ(I)> | 4.86 | 0.2 |
Completeness [%] | 98.9 | 86.2 |
Redundancy | 6.3 | 4.1 |
CC(1/2) | 0.994 | 0.338 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 20.5% PEG 3350, 0.4M MgCl2, 0.1M Bis-Tris pH 5.5 |